Coding

Part:BBa_K584021:Experience

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-17)


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Applications of BBa_K584021

iGEM2017 ASTWS-China replaced the coding sequence from 222aa to 1061aa of INP with a 6His tag flanked by BamHI and HindIII sites to make a new part BBa_K2513032(https://parts.igem.org/Part:BBa_K2509019). Further we added an NdeI site before the start codon and an XhoI site after the final stop codon in order that it could be easily ligated into pET expression vectors. This part can be used in the surface display of recombinant proteins or small tags on Escherichia coli by replacing 6His tag with a target protein with or without a stop codon.

User Reviews

UNIQ0bb069476de5ac93-partinfo-00000000-QINU UNIQ0bb069476de5ac93-partinfo-00000001-QINU